Which statement best contrasts Northern blot quantification with RT-qPCR for measuring RNA abundance?

• A. Northern blot detects RNA via hybridization to a labeled probe, while RT-qPCR quantifies cDNA with fluorescence and is more sensitive; use reference genes and proper controls.
• B. RT-qPCR does not quantify RNA.
• C. Northern blot provides higher sensitivity than RT-qPCR.
• D. Reference genes are unnecessary in RT-qPCR.

Answer: (A)