What best describes prime editing in comparison to standard CRISPR-Cas9 knockout?

• A. Prime editing uses Cas9 nickase and reverse transcriptase with a pegRNA to write precise edits without requiring DSBs.
• B. Prime editing relies on double-strand breaks and NHEJ.
• C. Prime editing deletes large genomic regions.
• D. Prime editing cannot target base substitutions.

Answer: (A)